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The mechanism underlying the reduction of potassium channels in the aged mammalian brain is not well understood. Recently, there has been considerable interest in the contribution of a new membrane-bound cellular stress protein, heme oxygenase-1 (HO-1) to this altered membrane permeability during aging. Because the brain contains both glial and neuronal cells, the distribution of HO-1 was studied in the hippocampus and the prefrontal cortex, the major regions associated with memory and spatial navigation. HO-1 immunoreactive neurons were identified in all the subfields of the hippocampus and in all layers of the cortex. Double immunostaining with HO-1 and glial fibrillary acidic protein (GFAP), a marker of astrocytes, was used to compare the distribution of HO-1 with that of GFAP in the hippocampus and the cortex. There was extensive overlap of immunoreactivity for HO- 0b46394aab
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